Administrator: Julia E. Richards, Ph.D.
Staff: David Reed, Ph.D.
Email: dmreed@umich.edu
Phone: 734-764-0059
Fax: 734-615-0542

Module Services
This Module facilitates and expedites vision research by providing an array of services involving microarray and molecular biology technologies. The facility also provides access to large equipment that would be impractical for a single lab to acquire or maintain.

• NEI-funded investigations
• Generation of pilot data for vision grant proposals
• Collaborative investigations involving vision research

Primary Services

• Execution and/or training of experimentation involving Module equipment and protocols
• Gene expression profiling and data analysis
• Microarray Data Storage (as needed)
• Maintenance/oversight of Module equipment

Experimentation/Protocols

• Affymetrix Gene Chip Arrays: Purchase, Hybridization, Scanning, and Analysis
• Custom cDNA Arrays: Design, Generation, Hybridization, Scanning and Analysis
• Labeling of Target RNAs for Microarray Experiments
• Gene Expression Data Analysis (Normalization and First Level Analysis)
• RNA Isolation (primarily for Microarray Experiments)
• Quantitative PCR
• qPCR/Cloning Primer Design
• Laser Capture Microdissection
• Baculovirus Recombinant Protein Expression
• Subcloning for specific projects
• Other molecular biology protocols, depending on availability

Module Equipment

2nd Floor

• Molecular Dynamics Storm 840 Phosphoimager
• Affymetrix GeneChip Scanner 3000
• Affymetrix 428 Array Scanner
• Affymetrix GeneChips Fluidic Station 450
• Kodak X-OMAT 1000A Film Developer
• Kodak Image Station 440CF
• US Filter Elga Purelab Plus Water Purification System
• Beckman J2-21M Centrifuge
• Beckman L8-70M Ultra Centrifuge
• Integrated SpeedVac System ISS110
• Tuttnauer-Brinckman 3870E Tabletop Autoclave

3rd Floor

• Arcturus AutoPix Laser Capture Microdissection

4th Floor

• Molecular Devices SpetraMax 190 Microplate Reader

5th Floor

• US Filter Elga Purelab Ultra Water Purification System
• Beckman DU530 Spectophotometer
• Perkin-Elmer Lambda Bio20 Spectrophotometer
• Thermo Spectronic French Press
• Beckman 5500 Gamma Counter
• Perkin-Elmer WinSpectral 1414 Scintillation Counter
• Integrated SpeedVac System ISS110
• Alpha Innotech Image Station
• Affymetrix GeneChip Hybridization Over 640
• Turner BioSystems Veritas Microplate Luminometer
• Amaxa Nucleofactor II Transfection System
• Beckman Optima L-90K Ultra Centrifuge
• Kodak Image Station 440CF
• Virtek SDDC-2 Robotic Microarrayer

8th Floor

• Amsco 2031 Autoclave
• Steris Basil 3500 Cage and Bottle Washer